Laser Scanning Confocal Microscope Magnification

Relatively thick specimens can be imaged in successive volumes by acquiring a series of sections along the optical z axis of the microscope.

Laser scanning confocal microscope magnification.

The laser scans across the object and an image is built up pixel by pixel on a screen. In the past the traditional laser microscope excited the whole thickness of the sample resulting in saturated blurry images and sometimes visualizing false colocalization images. Laser scanning confocal microscopy laser scanning confocal microscopes employ a pair of pinhole apertures to limit the specimen focal plane to a confined volume approximately a micron in size. Confocal microscopy most frequently confocal laser scanning microscopy clsm or laser confocal scanning microscopy lcsm is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out of focus light in image formation.

A confocal laser scanning microscope or also known as laser scanning confocal microscope is used to obtain high resolution images and generate 3d reconstructions through direct optical sectioning to provide clear images from a range of depths of thicker specimens for nanometer level imaging and measurement. Fluorescent microscopy not only makes our images look good it also allows us to gain a better understanding of cells structures and tissue. Clsm combines high resolution optical imaging with depth selectivity which allows us to do optical sectioning. This means that we can view visual sections of tiny structures that.

The confocal laser scanning microscope s aim was not to further increase magnification but to make clearer.